Novel Role for Pendrin in Orchestrating Bicarbonate Secretion in Cftr-expressing Airway Serous Cells
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چکیده
NOVEL ROLE FOR PENDRIN IN ORCHESTRATING BICARBONATE SECRETION IN CFTR-EXPRESSING AIRWAY SEROUS CELLS James P Garnett, Emma Hickman, Rachel Burrows, Péter Hegyi, László Tiszlavicz, Alan W Cuthbert, Peying Fong and Michael A Gray Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.; Novartis Institutes for Biomedical Research (NIBR), Novartis Horsham Research Centre, Horsham, West Sussex, RH12 5AB, UK.; Department of Medicine, Department of Pathology, University of Szeged, Szeged, Hungary.; Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Cambridge CB2 2QQ, UK.; Department of Anatomy and Physiology, Kansas State University College of Veterinary Medicine, Manhattan, KS 66502, USA; Current address: Division of Biomedical Sciences, St George's, University of London, London, SW17 0RE, UK. Running title: Role of pendrin in HCO3 secretion in the airways Address correspondence to: Michael Gray, Ph.D., Epithelial Research Group, Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK. Tel: +44 (0) 191 222 7592. Fax: +44 (0) 191 222 7424. Email: [email protected]
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Most bicarbonate secretion by Calu‐3 cells is mediated by CFTR and independent of pendrin
Bicarbonate plays an important role in airway host defense, however, its transport mechanisms remain uncertain. Here we examined the relative contributions of the anion channel CFTR (cystic fibrosis transmembrane conductance regulator, ABCC7) and the anion exchanger pendrin (SLC26A4) to HCO3- secretion by the human airway cell line Calu-3. Pendrin and CFTR were both detected in parental Calu-3 ...
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